A chamber has been designed to enable growth and observation of microcolonies of fungi in isolation from the external environment.
Unlike prior fungus growing apparatuses, this chamber makes it possible to examine a fungus culture without disrupting it. Partly resembling a small picture frame, the chamber includes a metal plate having a rectangular through the thickness opening with recesses for a top and a bottom cover glass, an inlet for air, and an inlet for water. The bottom cover glass is put in place and held there by clips, then a block of nutrient medium and a moisture pad are placed in the opening. The block is inoculated, then the top cover glass is put in place and held there by clips. Once growth is evident, the chamber can be sealed with tape. Little (if any) water evaporates past the edges of the cover glasses, and, hence there is little (if any) need to add water. A microscope can be used to observe the culture through either cover glass. Because the culture is sealed in the chamber, it is safe to examine the culture without risking contamination. The chamber can be sterilized and reused.
This work was done by Duane L. Pierson of Johnson Space Center and Thomas C. Molina of KRUG Life Sciences. For further information, contact the Johnson Innovative Partnerships Office at (281) 483-3809. MSC-22904