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Technique for Reversible Permeabilization of Live Cells for Intracellular Delivery of Quantum Dots

Lyndon B. Johnson Space Center, Houston, Texas Nanomaterials are comparable in size to various biomolecules (1 to 100 nm), and have unique properties such as enhanced electrical conductivity, increased chemical reactivity, and novel optical properties that make them attractive candidates for various biomedical applications. Their comparable size and unique optical properties have been utilized to develop efficient tools for subcellular imaging and delivery of biomolecules. Traditional bimolecular delivery methods utilize plasmids, cationic polymers, lipids, and viruses that have inherent disadvantages such as degradation in physiological solutions, and the need for complex conjugation techniques.

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Introduction of Structural Affinity Handles as a Tool in Selective Nucleic Separations

Lyndon B. Johnson Space Center, Houston, Texas This invention relates to the general field of bio-chemical separations. Current methods for separating nucleic acids either lack sufficient selectivity or large-capacity production as required for gene therapy or DNA vaccines or other applications. There is a need for more selective methods of separating nucleic acids that results in a relatively uncontaminated product in larger volumes and yields than achieved with currently available separation methods.

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Functionalized Patterning of Tissue Engineering Substrates

Task also includes bioprinting cell-laden constructs for multi-compartment tissue chambers. Lyndon B. Johnson Space Center, Houston, Texas Tissue engineering (TE) is an emerging field for tissue repair and regeneration compared to conventional techniques including autograft and allograft, through engineering functional implants created from living cells. TE is a highly interdisciplinary research area where material science, engineering, and biology are blended to achieve tissue regeneration. Efforts have been made to regenerate liver, skin, bone, and vascular tissues by applying a tissue engineering approach. To generate any type of tissue in a laboratory environment, scientists need to mimic the cellular microenvironment by offering structural, chemical, physical, and biological cues to the cells. Introduction of these cues to the cellular environment starts with manufacturing a supportive matrix called a scaffold.

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Hearing Test System Suitable for Self-Administered Hearing Assessment

Lyndon B. Johnson Space Center, Houston, Texas Astronauts have developed hearing loss in space, so the goal of this project was to provide a robust, self-administered, accurate noise-tolerance hearing test system for astronauts. The device includes Bekesy-like threshold audiometry, distortion product otoacoustic emission testing, and gap-detection testing.

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Assessment of Microbial Bioburden Within Aerogel Matrices

A post-capture aerogel degradation via cryogenic grinding is compatible with downstream nucleic-acid-based molecular modes of analysis. NASA’s Jet Propulsion Laboratory, Pasadena, California A makeshift apparatus has been designed composed of a sealed, hydrophobic 2-propanol/SiO2 aerogel component to filter outside air particles. Following verification and assessment, the apparatus was crafted with a Buchner funnel. Aerogel matrices were tightly fitted into filter housings and secured in side-arm flasks, which were then equipped to a vacuum pump to pull air through the aerogel matrices. Aerogels, both with and without fiberglass reinforcement, were used to collect airborne particulates for one- and three-hour increments. An untreated negative control aerogel, employing air collection from a laminar hood, and a positive aerogel matrix were seeded with endospores that verified the extraction from the matrices.

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The Advanced Data Analytics Platform (ADAPT): Concept, Design, Architecture, and Operation

Goddard Space Flight Center, Greenbelt, Maryland NASA scientists are uniquely positioned to research and understand the processes affecting the Earth’s climate. To study these important processes, scientists must address the Big Data challenges posed by working with massive amounts of observational and climate model output data. The Advanced Data Analytics Platform (ADAPT) is a cyber infrastructure resource specifically designed to reduce the friction between scientists and data. The system includes a high-performance storage cloud surrounded by large-scale compute resources. A very high-performing network enables fast access to the data stored within ADAPT. Furthermore, the system allows users to bring their applications to the data and define the environment in which those applications run. The science results can then be stored for future analysis or shared through static and dynamic data services within ADAPT without having to move the data or make additional copies. The agility, flexibility, and extensibility of the system make it ideal for NASA scientists to produce science results quickly by analyzing large data sets.

Posted in: Briefs, TSP, Electronics & Computers

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Orion Heat Shield Thermal Mapping Tool

The Orion Heat Shield Mapping Tool collects data from a set of output files from various re-entry thermal response codes, interpolates the data, and maps the analysis code data onto a finely meshed finite element model.

Posted in: Briefs, Electronics & Computers

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