Instrument Measures Fluorescence From Chlorophyll in Plants
Stennis Space Center, Mississippi
Apr 30 2000
Page 1 of 2
Effective discrimination against solar background radiation is achieved without
critical optical and mechanical parts.
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An optoelectronic instrument, known
as a plant fluorescence sensor, is being developed for use as a working tool
in agricultural settings. This instrument is a remote, passive monitor that
provides a means of discerning plant stress at very early stages. With sufficient
warning, the user could provide timely applications of fertilizer, water, and/or
pesticide to achieve maximum crop yield at minimum cost. Figure 1 presents two
views of the plant fluorescence sensor. The instrument is the subject of U.
S. Patent 5,567,947.
Measurement of steady-state plant fluorescence offers the possibility
of determining the physiological status of a green plant. The magnitude of plant
fluorescence and its spectral (color) distribution is sensitive to a number
of factors which are related to the ability of a plant to perform photosynthesis
(the process by which green plants convert atmospheric water vapor and carbon
dioxide into sugars and oxygen, using sunlight as fuel). For instance, the light
capture efficiency of the plant is dependent on the type and amount of pigment
molecules (such as chlorophyll) which in turn is dependent on adequate fertilization.
Plants stressed from a lack of fertilizer will limit chlorophyll production
and exhibit both lower overall level of fluorescence and shift in spectral distribution
compared to healthy plants. Another factor, such as lack of adequate water,
can serve to limit the rate of photosynthesis by causing the plant to close
its stomata (the openings which allow the leaf to draw in carbon dioxide and
water vapor); when this happens, the level of plant fluorescence will generally
increase. Thus, measurements taken with this sensor can guide growers in the
allocation of resources such as irrigation water, fertilizer, and pesticides.
Figure 1. The Plant Fluorescence Sensor in the left view is lying, with its casing removed, on a utility cart. In the right view, it is installed on a high-clearance vehicle for monitoring a Nebraska corn field.
Under sunlight, the chlorophyll in plants fluoresces at wavelengths
from about 660 to 800 nm. The major problem in measuring this fluorescence is
to discriminate against scattered sunlight, which can contribute a spurious
component to the measurement. The present sensor is of the class of apparatuses
known as Fraunhofer-line or spectral-line discriminators, but this sensor differs
from others of its class by virtue of a unique design that exploits the spectral
absorption lines of oxygen in such a way as to obtain enhanced spectral discrimination
at lower cost. The desired spectral resolution and discrimination are achieved
without need for the highly precise, expensive optical components with critical
mechanical adjustments (e.g., Fabry-Perot cavities) that are used in other spectral-line
discriminators.
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