Tech Briefs

The benefits of two-photon fluorescence microscopy are realized at reduced cost.

The two-photon microscope is now being employed to study osteoblast and osteoclast bone cells in cultures, including the effects of fluid flow and other environmental stimuli. Ultimately, these studies will be used to investigate and develop effective countermeasures to the bone loss experienced in a reduced- gravity environment. In addition, a variant of this instrument is being considered as an add-on module to the Light Microscopy Module, which will be deployed on the International Space Station.

This work was done by David G. Fischer and Gregory A. Zimmerli of Glenn Research Center and Marius Asipauskas of the National Center for Microgravity Research.

Inquiries concerning rights for the commercial use of this invention should be addressed to NASA Glenn Research Center, Commercial Technology Office, Attn: Steve Fedor, Mail Stop 4–8, 21000 Brookpark Road, Cleveland, Ohio 44135. Refer to LEW-17573-1.

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