Spectra-Physics’ (Mountain View, CA) newest addition to the Mai Tai Ti:Sapphire laser product line delivers more than 2.5 W of average power and is now available with a 700-1020 nm tuning range. The laser is fully computer controlled and produces 100 femtosecond pulses. With a broader tuning range, the laser enables two-photon excitation of all commonly used fluorophores, including the fluorescent proteins. The high power makes the laser applicable for other non-linear spectroscopy applications where pulse energy is important, while enabling deeper tissue penetration in biophotonics applications. Each Mai Tai laser features automated wavelength tuning for real-time excitation profiling, as well as pointing stability that eliminates the need to realign the experimental setup.