Researchers have developed a new method of 3D-printing gels and other soft materials that has the potential to create complex structures with nanometer-scale precision. Because many gels are compatible with living cells, the new method could jump-start the production of soft, tiny medical devices, such as drug delivery systems or flexible electrodes, that can be inserted into the human body.

Illustration of a prospective biocompatible interface shows that hydro-gels (green tubing), which can be generated by an electron or X-ray beam 3D printing process, act as artificial synapses or junctions connecting neurons (brown) to electrodes (yellow). (Credit: A. Strelcov/NIST)

A standard 3D printer makes solid structures by creating sheets of material — typically plastic or rubber — and building them up, layer by layer, until the entire object is created. In the standard method, the 3D printer chamber is filled with long-chain polymers — long groups of molecules bonded together — dissolved in water. Then, special molecules that are sensitive to light are added. When light from the 3D printer activates those special molecules, they stitch together the chains of polymers so that they form a fluffy, weblike structure. This scaffolding, still surrounded by liquid water, is the gel.

Typically, modern 3D gel printers use ultraviolet or visible laser light to initiate formation of the gel scaffolding; however, the researchers focused their attention on a different 3D-printing technique to fabricate gels using beams of electrons or X-rays. Because these types of radiation have a higher energy, or shorter wavelength, than ultraviolet and visible light, these beams can be more tightly focused and therefore produce gels with finer structural detail. Such detail is exactly what is needed for tissue engineering and many other medical and biological applications. Electrons and X-rays offer a second advantage: They do not require a special set of molecules to initiate the formation of gels.

At present, the sources of this tightly focused, short-wavelength radiation — scanning electron microscopes and X-ray microscopes — can only operate in a vacuum where the liquid in each chamber evaporates instead of forming a gel. The researchers demonstrated 3D gel printing in liquids by placing an ultrathin barrier — a thin sheet of silicon nitride — between the vacuum and the liquid chamber. The thin sheet protects the liquid from evaporating (as it would ordinarily do in vacuum) but allows X-rays and electrons to penetrate into the liquid. The method enabled the team to use the 3D printing approach to create gels with structures as small as 100 nanometers or about 1,000 times thinner than a human hair. By refining their method, the researchers expect to imprint structures on the gels as small as 50 nm, the size of a small virus.

Some future structures made with this approach could include flexible injectable electrodes to monitor brain activity, biosensors for virus detection, soft microrobots, and structures that can emulate and interact with living cells and provide a medium for their growth.

For more information, contact Andrei Kolmakov at andrei. This email address is being protected from spambots. You need JavaScript enabled to view it.; 301-975-4724.


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This article first appeared in the May, 2021 issue of Tech Briefs Magazine.

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