A team of researchers at the Department of Energy’s BioEnergy Science Center (BESC) have pinpointed the gene that controls ethanol production capacity in a microorganism. This discovery could be the missing link in developing biomass crops that produce higher concentrations of ethanol at lower costs.
The discovery of the gene Clostridium thermocellum will mean that scientists can experiment with genetically altering biomass plants to produce more ethanol. Current methods to make ethanol from a type of biomass found in switchgrass and agricultural waste require the addition of expensive enzymes to break down the plant’s barriers that guard energy-rich sugars.
Scientists, including those at BESC, have been working to develop a more streamlined approach in which tailor-made microorganisms produce their own enzymes that unlock the plant’s sugars and ferment them into ethanol in a single step. Identifying this gene is a key step towards making the first tailor-made microorganism that produces more ethanol.
Although scientists have studied Clostridium thermocellum for decades, the genetic basis for its ability to tolerate higher concentrations of ethanol had not been determined. Rather than using just one technique or one approach, the research team that made the discovery was able to draw upon multiple experts spanning several scientific disciplines to contribute a broader set of analyses because of the BESC partnership.