An innovative microfabrication technique used to produce a mirror array that both directs the excitation beam and holds the sample may revolutionize the field of 3D super-resolution mi - croscopy. The Single-Objective Selective-Plane Illumination Microscopy (soSPIM) allows re - searchers to examine the activity of single proteins or entire embryos on their existing microscope systems. The soSPIM technique utilizes an array of micromirrored wells, where each mirror is inclined at precisely 45 degrees. Together with a beam steering add-on unit, these micromirrors allow for both the excitation beam and resulting fluorescence signal to pass through a single standard, objective lens.
It was found that soSPIM’s acquisition rate was limited solely by sample brightness and the camera’s maximum acquisition rate, so the researchers used an Andor (Belfast, UK) Neo 5.5 sCMOS camera that had proved reliable in the past. Its large, 22-mm diameter, 5.5-megapixel sensor is ideal for cell microscopy, and the frame rate of 30 fps sustained or 100 fps burst mode coped easily with the task.
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