An apparatus denoted a swab device has been developed as a convenient means of acquiring samples of contaminants from surfaces and suspending the samples in liquids. (Thereafter, the liquids can be dispensed, in controlled volumes, into scientific instruments for analysis of the contaminants.) The swab device is designed so as not to introduce additional contamination and to facilitate, simplify, and systematize the dispensing of controlled volumes of liquid into analytical instruments.
The use of currently commercially available contamination-sampling devices involves significant mechanical manipulation of samples and liquids, and there is no provision for systematic dispensing of controlled volumes of liquid into analytical instruments: A typical use involves wiping a surface of interest with a standard implement resembling a cotton swab. The implement is then placed into a volume containing the liquid in which the sample is to be suspended. Ultimately, the liquid must be extracted from this volume and dispensed into an analytical instrument by use of a pipette. The swab device is a single apparatus into which are combined all the equipment and materials needed for sampling surface contamination. The swab device contains disposable components stacked together on a non-disposable dispensing head. One of the disposable components is a supply cartridge holding a sufficient volume of liquid for one complete set of samples. (The liquid could be clean water or another suitable solvent, depending on the application.) This supply of liquid is sealed by Luer valves.
At the beginning of a sampling process, the user tears open a sealed bag containing the supply cartridge. A tip on the nondisposable dispensing head is engaged with a Luer valve on one end of the supply cartridge and rotated, locking the supply cartridge on the dispensing head and opening the valve. A bag containing a disposable swab tip is opened, and the end of the supply cartridge opposite the aforementioned end is engaged with the swab tip and rotated, opening a valve.
The swab tip includes a fabric swab that is wiped across the surface of interest to acquire a sample. A sealed bag containing a disposable dispensing tip (not to be confused with the non-disposable dispensing head) is then opened, and the swab tip is pushed into the dispensing tip until seated. The dispensing head contains a piston that passes through a spring-loaded lip seal. The air volume displaced by this piston forces the liquid out of the supply cartridge, over the swab, and into the dispensing tip. The piston is manually cycled to enforce oscillation of the air volume and thereby to cause water to flow to wash contaminants from the swab and cause the resulting liquid suspension of contaminants to flow into the dispensing tip. After cycling several times to ensure adequate mixing, liquid containing the suspended contaminant sample is dispensed through the dispensing tip in 25- μL increments into an analytical instrument. The disposable components are then removed from the dispensing head. Thereafter, the dispensing head can be reused with a fresh set of disposable components.