Researchers funded by the National Institute of Biomedical Imaging and Bioengineering (NIBIB) have developed a non-invasive imaging technique that accurately detects skin cancer without surgical biopsy. Multiphoton microscopy of mitochondria — small organelles that produce energy in cells — accurately identified melanomas and basal cell carcinomas by detecting abnormal clusters of mitochondria in both types of skin cancer.

Skin cancer is the most common type of cancer in the U.S., and most types of skin cancer are highly treatable, especially if detected early. Rather than taking a biopsy sample that must be processed and then examined under a microscope by a pathologist, this system involves simply looking through the microscope at the patient's skin and determining whether it is cancerous or not, within minutes.

This diagram shows differences that can be observed in cell morphology in normal skin cells versus melanomas. Pink images show differences following biopsy and staining by a pathologist. Green slices show differences in fluorescence patterns of mitochondria using multiphoton microscopy. (Irene Georgakoudi, Tufts University)

International researchers found that mitochondria behave very differently in healthy versus cancerous tissue. They used a laser microscopy technique that takes advantage of the characteristics of a key molecule in mitochondria — nicotinamide adenine dinucleotide (NADH) — that is central to energy production. They found that NADH, which naturally fluoresces without injecting any dye or contrast agent into the individuals being screened, can be detected using multi-photon microscopy to provide diagnostically useful information about the organization of the mitochondria in skin cells.

The system enables users to obtain very high-resolution images of individual cells without having to slice the tissue physically. With the technique, it was found that in normal cells, the mitochondria are spread throughout the cell in a web-like pattern. Conversely, cancerous skin cells show a very different pattern, with the mitochondria found in clumps or clusters typically at the center of the cell along the border of the nucleus.

In this study, the technique was tested in ten patients with skin cancer (melanoma or basal carcinoma) and four who did not have skin cancer. The imaging technique results were compared to the traditional biopsy results obtained from each patient. The results demonstrated that the imaging technique correctly identified skin cancer in all ten cancer patients, and made no false diagnoses in the four individuals without skin cancer.

It is estimated that this test could be routinely used in doctor's offices within five years, although the cost for the laser used in this microscope could limit the medical facilities that would be able to make such an investment. Less-expensive lasers are on the horizon; however, this approach would enable a doctor to make a quick diagnosis and begin treatment immediately, which could ultimately lower healthcare costs associated with these very common cancers.

For more information, contact Thomas Johnson at This email address is being protected from spambots. You need JavaScript enabled to view it.; 301-496-3500.


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This article first appeared in the January, 2019 issue of Tech Briefs Magazine.

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