A proposed biosensing system would be based on a combination of (1) a sensing volume containing antibodies immobilized in a sol-gel matrix and (2) an optical interferometer having a ring resonator configuration. The antibodies would be specific to an antigen species that one seeks to detect. The binding of the antigens to the immobilized antibodies would change the index of refraction of the sensing volume, which would be mounted in one of the interferometer arms. The interferometer would measure the change in the index of refraction, thereby indirectly measuring the concentration of the antigen species of interest.
The basic principle of using interferometry to detect antibody-antigen binding is not new in itself. However, the prior implementation of this principle has involved the use of a Mach-Zehnder interferometer, which affords only a single pass of light through the sensing volume. In the ring resonator of the proposed system, light would make multiple passes through the sensing volume, affording greater interaction length and, hence, greater antibody-detection sensitivity.
In one proposed ring-resonator/interferometer configuration, there would be two interferometer arms with coupled optical paths. One of the optical paths would pass through the sensing volume; the other optical path would not pass through the sensing volume (see figure). Interference between light beams in the two interferometer arms would be characterized by a phase difference proportional to the change in the index of refraction of the sensing volume. The phase difference would result in a change in the interferometer output intensity measured by use of a photodiode. A synchronous detector could be used to increase sensitivity.
The ring resonator/interferometer could be built by use of traditional bulk optical components or fabricated as a unit by standard silicon-fabrication techniques. Inasmuch as a sol-gel precursor can be poured into a mold, an etched recess in a planar waveguide or other structures could be used as the sensing volume.
This work was done by Gregory Bearman and David Cohen of Caltech for NASA's Jet Propulsion Laboratory. For more information, download the Technical Support Package (free white paper) at www.techbriefs.com/tsp under the Physical Sciences category.
This invention is owned by NASA, and a patent application has been filed. Inquiries concerning nonexclusive or exclusive license for its commercial development should be addressed to the Patent Counsel, NASA Management Office–JPL. Refer to NPO-30807.